USP14
- 貨號 Cat. No.
- E-544
- 規格 Size
- 25 μg
- 品牌 Brand
- Boston Biochem
Description
USP14
human recombinant
Product Information
25 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50mM NaCl, 5mM DTT, 1mM EDTA. Actual concentration varies with specific Lot #.
56 kDa
> 95% by SDS-PAGE
Use & Storage
Activity was measured using 18 μM Enzyme, 4 μM Ub-AMC in 100mM Hepes pH8.5, 10mM DTT and 5% glycerol at 25°C.
Under these conditions RFU/min/μg = 2.0
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-544
USP14
Product Source:
human recombinant
USP14 (Ubiquitin Specific Protease 14) is the human homolog of the yeast Ubp6 protein and is member a of the family of thiol proteases involved in the hydrolysis of ubiquitin C-terminal protein derivatives. It has been reported to have a low-affinity association with the proteasome (PA700, 19S cap particle), which increases its basal enzyme activity. The primary physiological role of USP14 may be the release of ubiquitin from proteosome-bound conjugates. In vitro, the enzyme has a low affinity for ubiquitin and is unable to disassemble poly-ubiquitin protein conjugates. Mice homozygous for spontaneous USP14 mutations develop ataxia and this enzyme may play an important role in regulating synaptic activity in mammals. USP14 may also have a role in colorectal cancer metastases, possibly controlling the fate of proteins that regulate tumor invasion. Accession # NP_005142.
Product Information
Quantity:
25 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50mM NaCl, 5mM DTT, 1mM EDTA. Actual concentration varies with specific Lot #.
MW:
56 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Activity was measured using 18 μM Enzyme, 4 μM Ub-AMC in 100mM Hepes pH8.5, 10mM DTT and 5% glycerol at 25°C.
Under these conditions RFU/min/μg = 2.0
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.