UFM1 Conjugation Initiation Kit
- 貨號 Cat. No.
- K-500
- 規格 Size
- 1 kit
- 品牌 Brand
- Boston Biochem
Description
UFM1 Conjugation Initiation Kit
human recombinant
Product Information
1 kit
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: K-500
UFM1 Conjugation Initiation Kit
Product Source:
human recombinant
This kit is designed for the conjugation of the ubiquitin-like modifier Ufm1 to protein substrates in vitro, which requires the activities of the human Ufm1 E1 (E-319) activating enzyme and the Ufc1 (E2-675) E2 enzyme. The E1 enzyme charges the Ufm1 by forming an ATP-dependent high energy thiolester bond. The activated UFm1 is
subsequently transferred to Ufc1 and this E2-S-Ufm1 thiolester complex can be used for the conjugation of Ufm1 to protein substrates with the addition of necessary E3 enzymes (not supplied).
NOTE: Kit contains reagents sufficient for 10 x 20 μl reactions. All protein are Nterminally His6-tagged.
Product Information
Quantity:
1 kit
Supplied:
| Concentration | Volume | |
| 20X Ufm1 E1 Enzyme | X mg/ml (X μM) | 10 μl |
| 20X Ufm1 E1 Enzyme | X mg/ml (X μM) | 20 μl |
| 10X Ufm1 | X mg/ml (X μM) | 20 μl |
| 10X Mg-ATP Solution | X mM | 20 μl |
| 10X Reaction Buffer | 500 mM Hepes pH 8.0 | 20 μl |
| 500 mM NaCl |
Background:
The Ufm1 E1 enzyme uses ATP to adenylate the C-terminal glycine residue of Ufm1, forming a high-energy thiolester bond. The second step is the trans-esterification reaction whereby the activated Ufm1 is transferred to the active site cysteine of Ufc1. Ufc1 is a member of the E2 family and is homologous to ubiquitin-conjugating enzymes, but is specific for the conjugation of Ufm1 to a variety of target proteins. The Ufm1 pathway is distinct from ubiquitination with different substrate specificity and interaction with ligating enzymes. It is known that Ufm1 becomes conjugated to as yet unidentified proteins in HEK cells and various mouse tissues. Ufm1 is conserved in metazoa and plants, but not yeast. The exact cellular function and role of Ufm1 modification in vivo is not yet known.
Use & Storage
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.