UCHL1 Protein, Human, Recombinant (His Tag)

Purity

> 92 % as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Activity

Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/μg.

Protein Construction

A DNA sequence encoding the native human UCHL1 (NP_004172.2) (Gln 2-Ala 223) was expressed, with a polyhistide tag at the N-terminus.

NP#

Expressed Host

E. coli

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human UCHL1 consisting of 229 amino acids and migrates as an 25.6 kDa band in SDS-PAGE under reducing conditions as predicted.

Formulation

Lyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, 1mM DTT, pH 8.0

1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

2. Please contact us for any concerns or special requirements.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -70℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.