UCH-L3

貨號 Cat. No.
E-325
規格 Size
25 μg
品牌 Brand
Boston Biochem

Description

UCH-L3

Catalog #: E-325




UCH-L3






Product Source: 

human recombinant



Also known as: Ubiquitin C-terminal Hydrolase L3 (UCH-L3)


Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters from the C-terminus of ubiquitin. UCH-L3 is a member of the low molecular weight UCHs that processes ubiquitin precursors and ubiquitinated proteins to generate monomeric ubiquitin. This enzyme is also able to cleave at the C-terminus of NEDD8. UCH-L3, like UCH-L3 may have roles in the maintenance of neurons in the gracile tract, nucleus tractus solitaris and area postrema.


Product Information



Quantity: 

25 μg






Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.6, 1 mM DTT. Actual concentration will vary with specific Lot #.






MW: 

26 kDa






Purity: 

> 95% by SDS-PAGE




 


Use & Storage



Use: 

Typical enzyme concentration to support hydrolysis of substrates in vitro is 0.05-5 nM depending on conditions and substrate. Pre-incubation for 15 minutes with 10 mM DTT is recommended to achieve maximum activity.






Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.



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