Ubiquitin Vinyl Sulfone

貨號 Cat. No.
U-202
規格 Size
50 μg
品牌 Brand
Boston Biochem

Description

Ubiquitin Vinyl Sulfone

Catalog #: U-202




Ubiquitin Vinyl Sulfone






Product Source: 

human recombinant



Also known as: (Ub-VS)


A potent, irreversible and specific inhibitor of all ubiquitin C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs) and deubiquitinating enzymes (DUBs). Useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.


Product Information



Quantity: 

50 μg






MW: 

8.6 kDa






Purity: 

> 98 % by HPLC.






Solubility: 

Soluble in aqueous buffer up to 5 mg/ml. For best results, pellet dry compound prior to reconstitution.






Activity: 

Ki = < 40 pM versus UCHL3






Supplied: 

This is a lyophilized powder




 


Use & Storage



Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions typical concentrations to fully inhibit UCHs, USPs or DUBs in vitro are 1-5 μM.






Storage: 

Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.



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