Ubiquitin triple mutant K29R, K48R, K63R
- 貨號 Cat. No.
- UM-3KTR
- 規格 Size
- 1 mg
- 品牌 Brand
- Boston Biochem
Description
Ubiquitin triple mutant K29R, K48R, K63R
human recombinant
Product Information
1 mg
Soluble and stable in aqueous buffers up to 5 mg/ml.
8.5 kDa
> 95% by PAGE
This is a lyophilized powder
Use & Storage
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.
Catalog #: UM-3KTR
Ubiquitin triple mutant K29R, K48R, K63R
Product Source:
human recombinant
Mutation of lysines 29, 48 and 63 to arginine renders ubiquitin unable to form poly-ubiquitin chains via the major known poly-ubiquitination sites. The ubiquitin triple mutant can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for studies of alternate mono-ubiquitination or polyubiquitination sites.
Product Information
Quantity:
1 mg
Stock:
Soluble and stable in aqueous buffers up to 5 mg/ml.
MW:
8.5 kDa
Purity:
> 95% by PAGE
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Storage:
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.