Ubiquitin Rhodamine110
- 貨號 Cat. No.
- U-555
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Ubiquitin Rhodamine110
human recombinant
Product Information
50 μg
X mg/ml (XμM) in DMSO. Concentration varies with lot number.
8.9 kDa
> 95%
Use & Storage
μM,Ub-R110 gives a strong signal in the range of 0.1-1 depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex485 nm and Em535 nm wavelengths respectively.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: U-555
Ubiquitin Rhodamine110
Product Source:
human recombinant
Also known as: Ub-R110
Fluorogenic substrate for deubiquitinating enzymes based on the C-terminal derivative of ubiquitin with Rhodamine110 (R110). Similar to other C-terminus derivatives such as Ub-AMC (U-550) and Ub-AFC (U-551), this is an exquisitely sensitive deubiquitinating enzyme substrate and is useful for studying ubiquitin C-terminal hydrolytic activity when detection sensitivity or continuous monitoring of activity at longer wavelengths is essential.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (XμM) in DMSO. Concentration varies with lot number.
MW:
8.9 kDa
Purity:
> 95%
Activity:
UCH-L3 Km = 0.034 μM, USP2CD Km = 1.5 μM.
Use & Storage
Use:
μM,Ub-R110 gives a strong signal in the range of 0.1-1 depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex485 nm and Em535 nm wavelengths respectively.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.