Ubiquitin-R 74

Catalog #: UM-R74

Mature forms of ubiquitin have a highly conserved diglycine motif at the carboxyl terminus which is crucial for activity and recognition in conjugation and deconjugation reactions. The removal of this diglycine peptide (Ub ending in Arg⁷⁴) results in an inactive Ub. This Ub cannot be activated by the E1 enzyme in an ATP-dependent manner, is not capable of subsequent thioester interaction with E2 and/or E3 enzymes, and is thus not capable of forming isopeptide bonds or Ub conjugates. It can be used as a negative control in conjugation reactions, or in binding studies with E1, E2, E3 and DUB enzymes or other proteins that interact with ubiquitin via ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs).