Ubiquitin-Protein Conjugation Kit
- 貨號 Cat. No.
- K-960
- 規格 Size
- 1 kit
- 品牌 Brand
- Boston Biochem
Description
Ubiquitin-Protein Conjugation Kit
rabbit
Product Information
1 kit
Use & Storage
Store solutions at -80°C. Once thawed, keep on ice prior to use. For best results, aliquot and freeze after first set of experiments to avoid multiple freeze/thaw cycles.
Catalog #: K-960
Ubiquitin-Protein Conjugation Kit
Product Source:
rabbit
This kit is for the formation of ubiquitinated substrate proteins. The enzymes supplied represent the full compliment of purified conjugation enzymes (E1, E2s, and E3s) that are found in Fraction II. Conjugation Fraction A contains predominantly E1 and E2 enzymes, while Conjugation Fraction B contains predominantly E3 and deubiquitinating enzymes. The enzymes have been tested and shown to work with typical [125I]-labeled substrate proteins such as lysozyme and β- lactoglobulin. This conjugation fraction also contains ubiquitin C-terminal hydrolases and therefore the addition of Ubiquitin Aldehyde (U-201) is recommended for the inhibition of UCHs to improve overall conjugate yield. The supplied fractions do not contain 20S or 26S proteasomes or any other protein degradation activity. If the substrate being conjugated requires E2 or E3 enzymes not found in Fraction II, the reaction can be supplemented with enzymes from Fraction I (F-375).
NOTE: Kit contains reagents sufficient for 10 x 50 μl reactions.
Product Information
Quantity:
1 kit
Stock:
1. Conjugation Fraction A - X μg/ml, X μl (200 μg total)
In Buffer: 50 mM HEPES, pH 8.0, 0.5 mM DTT
2. Conjugation Fraction B - X μg/ml, X μl (200 μg total)
In Buffer: 50 mM HEPES, pH 8.0, 0.5 mM DTT
3. Ubiquitin - X mg/ml, 50 μl
4. Energy Solution - 10X Stock, 150 μL
Use & Storage
Storage:
Store solutions at -80°C. Once thawed, keep on ice prior to use. For best results, aliquot and freeze after first set of experiments to avoid multiple freeze/thaw cycles.