Ubiquitin K48R
- 貨號 Cat. No.
- UM-K48R
- 規格 Size
- 1 mg
- 品牌 Brand
- Boston Biochem
Description
Ubiquitin K48R
human recombinant
Product Information
1 mg
Soluble and stable in aqueous buffers up to 10 mg/ml.
8.5 kDa
> 95% by PAGE
This is a lyophilized powder
Use & Storage
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.
Catalog #: UM-K48R
Ubiquitin K48R
Product Source:
human recombinant
Mutation of lysine 48 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 48 linkages with other Ub molecules. Ub K48R can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (monoubiquitination). Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K48 linked.
Product Information
Quantity:
1 mg
Stock:
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW:
8.5 kDa
Purity:
> 95% by PAGE
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Storage:
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.