Ubiquitin AMC
- 貨號 Cat. No.
- U-550
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Ubiquitin AMC
human recombinant
Product Information
50 μg
X mg/ml (X μM) in 100 % DMSO Concentration varies with lot number.
8.6 kDa
> 95% by HPLC
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: U-550
Ubiquitin AMC
Product Source:
human recombinant
Fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Ubiquitin-AMC is an exquisitely sensitive substrate for UCH-L3 (Km = 0.039 μM) and for the Isopeptidase-T (Km = 0.17 - 1.4 μM). Ub-AMC is useful for studying ubiquitin hydrolases when detection sensitivity or continuous monitoring of activity is essential.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 100 % DMSO Concentration varies with lot number.
MW:
8.6 kDa
Purity:
> 95% by HPLC
Use & Storage
Use:
Substrate concentrations for assay range from 0.1-1 μM. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.