Ubiquitin AFC
- 貨號 Cat. No.
- U-551
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Ubiquitin AFC
human recombinant
Product Information
50 μg
X mg/ml (X μM) in 100 % DMSO Concentration varies with lot number.
8.6 kDa
> 95% by HPLC
Use & Storage
Substrate concentrations for assay range from 0.1-1 μM depending on assay conditions. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AFC fluorescence can be monitored using Ex400 nm and Em505 nm wavelengths, respectively.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: U-551
Ubiquitin AFC
Product Source:
human recombinant
Fluorogenic substrate based on the C-terminus derivatization of ubiquitin with 7-amino-4- trifluoromethylcoumarin (AFC). Similar to ubiquitin-AMC, this is an exquisitely sensitive deubiquitinating enzyme substrate and is useful for studying ubiquitin C-terminal hydrolytic activity when detection sensitivity or continuous monitoring is essential. The fluorophore has a larger Stokes radius than AMC which is useful to reduce compound interference in HTS assays.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 100 % DMSO Concentration varies with lot number.
MW:
8.6 kDa
Purity:
> 95% by HPLC
Use & Storage
Use:
Substrate concentrations for assay range from 0.1-1 μM depending on assay conditions. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AFC fluorescence can be monitored using Ex400 nm and Em505 nm wavelengths, respectively.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.