Ubiquitin Activating Enzyme (UBE1)
- 貨號 Cat. No.
- E-305
- 規格 Size
- 25 μg
- 品牌 Brand
- Boston Biochem
Description
Ubiquitin Activating Enzyme (UBE1)
human reconbinant
Product Information
25 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number
110 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Store at –80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-305
Ubiquitin Activating Enzyme (UBE1)
Product Source:
human reconbinant
Also known as: UBA1
This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its C-terminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions.
Product Information
Quantity:
25 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number
MW:
110 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Storage:
Store at –80°C. Avoid multiple freeze/thaw cycles.