Ubiquitin Activating Enzyme (UBE1)
- 貨號 Cat. No.
- E-301
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Ubiquitin Activating Enzyme (UBE1)
yeast
Product Information
50 μg
X mg/ml (X μM) in 50 mM HEPES pH8.0. Concentration varies with Lot #.
110 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is
Catalog #: E-301
Ubiquitin Activating Enzyme (UBE1)
Product Source:
yeast
This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its C-terminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester and free AMP. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. E1 is a critical component for the initiation of any in vitro conjugation reactions.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH8.0. Concentration varies with Lot #.
MW:
110 kDa
FW:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is