Ubiquitin
- 貨號 Cat. No.
- U-100Sc
- 規格 Size
- 5 mg
- 品牌 Brand
- Boston Biochem
Description
Ubiquitin
S.cerevisiae
Product Information
5 mg
8.6 kDa
> 95% by SDS-PAGE
Aqueous solutions up to 20 mg/ml
This is a lyophilized powder
Use & Storage
Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions
Lyophilized powder at 4 degrees C. Solubilized stock solution at -20 degrees C. Avoid multiple freeze/thaw cycles.
Catalog #: U-100Sc
Ubiquitin
Product Source:
S.cerevisiae
Highly purified ubiquitin free of glycine and buffer salts which can interfere with chemical and in vitro reactions. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic eukaryotic protein. It is covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteasome Pathway (UPP). The major role of ubiquitination is to target cellular proteins for the ATP-dependent degradation by the 26S proteasome and proteasome-independent or regulatory events such as protein localization, activity and function. This protein sequence is for S.cerevisiae (P61864) and is exactly the same for S.pombe.
Product Information
Quantity:
5 mg
MW:
8.6 kDa
Purity:
> 95% by SDS-PAGE
Solubility:
Aqueous solutions up to 20 mg/ml
Supplied:
This is a lyophilized powder
Background:
Protein Sequences:
H.sapiens Ub: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRT LSDYNIQKESTLHLVLRLRGG
S.cerevisiae Ub: MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRT LSDYN IQKESTLHLVLRLRGG
Use & Storage
Use:
Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions
Storage:
Lyophilized powder at 4 degrees C. Solubilized stock solution at -20 degrees C. Avoid multiple freeze/thaw cycles.