Ubiquitin
- 貨號 Cat. No.
- U-100At
- 規格 Size
- 5 mg
- 品牌 Brand
- Boston Biochem
Description
Ubiquitin
Product Information
Use & Storage
Catalog #: U-100At
Ubiquitin
Product Source:
A.thaliana
Highly purified ubiquitin free of glycine and buffer salts which can interfere with chemical and in vitro reactions. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic eukaryotic protein. It is covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteasome Pathway (UPP). The major role of ubiquitination is to target cellular proteins for the ATP-dependent degradation by the 26S proteasome and proteasome-independent or regulatory events such as protein localization, activity and function. This protein sequence is for A.thaliana (P59263) and is exactly the same for barley, oat, soya bean and other plants.
Product Information
Quantity:
5 mg
MW:
8.6 kDa
Purity:
> 95% by SDS-PAGE
Solubility:
Aqueous solutions up to 20mg/ml
Supplied:
This is a lypphilized powder
Background:
H.sapiens Ub: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRT
LSDYNIQKESTLHLVLRLRGG
A.thalianaUb:MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN IQKESTLHLV LRLRGG
Use & Storage
Use:
Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions
Storage:
Lyophilized powder at 4oC. Solubilized stock solution at -20oC. Avoid multiple freeze/thaw cycles.