UbcH9, Dominant Negative
- 貨號 Cat. No.
- E2-646
- 規格 Size
- 100 μg
- 品牌 Brand
- Boston Biochem
Description
UbcH9, Dominant Negative
human recombinant
Product Information
100 μg
X mg/ml (X μM) in 50 mM HEPES pH 7.6, 125 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.
20 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E2-646
UbcH9, Dominant Negative
Product Source:
human recombinant
UbcH9 is a member of the E2 family and is homologous to ubiquitin-conjugating enzymes, but is specific for the conjugation of SUMO to a variety of target proteins. This E2 is unusual in that it interacts directly with protein substrates that are modified by SUMOylation, and may play a role in substrate recognition. UbcH9 can mediate the conjugation of all SUMOs to a variety of proteins including RanGAP1, IκBα, and PML without the requirement of an E3 ligase. This enzyme has a mutation of the active site from cysteine to serine which abolishes the ability of UbcH9 to transfer ubiquitin to an accepting E3 protein. Ideal for use as a negative or competitive control, or to study protein-protein interactions.
Product Information
Quantity:
100 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 7.6, 125 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.
MW:
20 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.