UbcH7, Dominant Negative
- 貨號 Cat. No.
- E2-642
- 規格 Size
- 100 μg
- 品牌 Brand
- Boston Biochem
Description
UbcH7, Dominant Negative
human recombinant
Product Information
100 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.
18 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E2-642
UbcH7, Dominant Negative
Product Source:
human recombinant
UbcH7 mediates the selective degradation of short-lived and abnormal proteins and is highly homologous to UbcH5. UbcH7 interacts with the HECT domain of E6-AP and the RING domain of c-Cbl, and can mediate the multi-ubiquitination of many different types of protein substrates. This enzyme has a mutation of the active site from cysteine to serine which abolishes the ability of UbcH7 to transfer ubiquitin to an accepting E3 protein. Ideal for use as a negative or competitive control, or to study protein-protein interactions.
Product Information
Quantity:
100 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.
MW:
18 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.