UbcH5a, Dominant Negative
- 貨號 Cat. No.
- E2-618
- 規格 Size
- 100 μg
- 品牌 Brand
- Boston Biochem
Description
UbcH5a, Dominant Negative
human recombinant
Product Information
100 μg
[E2] = X mg/ml (X μM) in 50 mM HEPES pH 7.6, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.
17 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E2-618
UbcH5a, Dominant Negative
Product Source:
human recombinant
UbcH5 enzymes are human homologs of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. UbcH5a mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, IκBα, p105) and abnormal proteins. UbcH5a has 89% and 88% sequence identity with UbcH5b and UbcH5c respectively. This enzyme has an active site mutation from cysteine to serine which abolishes the ability of UbcH5a to transfer ubiquitin to an E3 protein. Ideal for use as a negative or competitive control, or to study protein-protein interactions.
Product Information
Quantity:
100 μg
Stock:
[E2] = X mg/ml (X μM) in 50 mM HEPES pH 7.6, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.
MW:
17 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.