UbcH12
- 貨號 Cat. No.
- E2-656
- 規格 Size
- 50 μg 100 μg
- 品牌 Brand
- Boston Biochem
Description
UbcH12
human recombinant
Product Information
50 μg 100 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 125 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.
20 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E2-656
UbcH12
Product Source:
human recombinant
also known as Ubc12,hUbc12, UBE2M
NEDDylation requires the coordinated activities of UbcH12 and the APPBPI/Uba3 activating enzyme. This E2 enzyme mediates the covalent conjugation of the ubiquitin-like protein NEDD8 to a limited number of cellular proteins such as Cdc53/Cul1 and other members of the cullin family (which are structural subunits of the Skp1-based and elongin B/C-based families of ubiquitin protein ligases). The NEDD8 pathway is essential for cell viability in fission yeast, and in mammalian cells it is essential for cell cycle progression and morphogenesis.
Product Information
Quantity:
50 μg 100 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 125 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.
MW:
20 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.