U-937
- 貨號 Cat. No.
- CLC036
- 品牌 Brand
- gene-rl
Description
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Cell Lines (GRL-CLC036) | |
Designations | U-937 |
| Cell type | Human histiocytic lymphoma |
Growth property | Suspension |
| Seeding density(cells/ml) | 2X106 |
Survival rate | 95% |
Culture medium | 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum |
| Incubation condition | 37°C，5% CO2 |
Freezed medium | 90% culture medium + 10% DMSO |
Medium renewal | Every 3-4 days |
| Subculturing | 。 Cultures can be maintained by addition or replacement of fresh medium. |
Mycoplasma test | Negative for bacteria，fungi and mycoplasma. |
Advertence: |
When you receive the cell line, please examine the flask and its cap. If it is not well-found, please tell us instantly. After examination, please wipe the flask and flask cap with 70% alcohol, and then loosen it. Afterward, put the flask into incubator immediately.