Tyrphostin AG 1478
- 貨號 Cat. No.
- T-320
- 規格 Size
- 10 x 0.1 mg 1mg 5x1mg
Description
Tyrphostin AG 1478
Potent EGF Receptor Tyrosine Kinase Inhibitor
| Product#: | T-320 |
| Sizes: |
| 10 x 0.1 mg |
| 1 mg |
| 5 x 1 mg |
| M.W.: | 315.76 |
| Structure: |
 |
| Purity: | >99% |
| Bioassay: |
| Tyrphostin AG 1478 inhibits EGF receptor activation. |
 |
3T3-L1 cells were serum starved for 3h and preincubated with different concentrations of Tyrphostin AG 1478 for 2h. The cells were then stimulated with 200ng/ml EGF for 10 min. The cell proteins were resolved by SDS- PAGE and detected with phospho-EGRr antibody (upper panel) or phospho-ERK1/2 antibody (lower panel). |
| References: |
1. | Zhu, X.F. et al. (2001) Cancer Lett. 169, 27. |
2. | Kauh, J.S. et al. (2003) Georgetown University Hospital, Washington, DC; Georgetown University, Washington, DC 2003 ASCO Annual Meeting Proc Am Soc Clin Oncol 22, 876 (abstr 3520). |
3. | Johns, T.G. et al. (2003) PNAS 100, 15871. |
4. | Choi, B.H. et al. (2002) Am. J. Physiol. Cell Physiol. 282, C1461 |
The Tyrphostin AG 1478 (AG 1478) is reversible, highly potent (IC50 = 3 nM) and selective inhibitor of EGFR tyrosine kinase activity, commonly use as an EGF signaling blocker.1
AG 1478 inhibits cell proliferation and arrests cell cycle in tumor cells with overexpression of EGFR.2,3
AG1478 also directly blocks (IC50 = 9.8 然) the rat brain Kv1.5 K+ channel in vitro in a non-protein-tyrosine-kinase-mediated manner.4