Antibodies
Tyrphostin 9
- 貨號 Cat. No.
- S2895
- 規格 Size
- 5~200mg
- 品牌 Brand
- Selleckchem
Description
Tyrphostin 9
Catalog No.S2895
Tyrphostin 9 is firstly designed as an EGFR inhibitor with IC50 of 460 μM, but is also found to be more potent to PDGFR with IC50 of 0.5 μM.
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Distributor Tel: +886-2-2827-1197jimmy@new.abscience.com.tw
Biological Activity
| Description | Tyrphostin 9 is firstly designed as an EGFR inhibitor with IC50 of 460 μM, but is also found to be more potent toPDGFR with IC50 of 0.5 μM. | |||||
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| Targets | EGFR | PDGFR | ||||
| IC50 | 460 μM [1] | 0.5 μM [6] | ||||
| In vitro | SF 6847 inhibits the replication of herpes simplex virus type 1 (HSV-1) with IC50 of 40 nM. SF 6847 (50 nM) partly reverses sodium orthovanadate induced HSV-1 plaque formation. SF 6847 (< 400 nM) decreases the phosphorylation of viral phosphoproteins in a dose-dependent manner, but the SF 6847 (< 800 nM)-induced reduction of protein synthesis is not dose-dependent. [2] SF 6847 prevents PDGF-induced tyrosine phosphorylation of LRP in caveolae in human fibroblasts suggesting that PDGF-BB-mediated LRP activation requires tyrosine phosphorylation and therefore activation of PDGFR-β. [3] SF 6847 (1 mM) blocks the strain-induced stimulatory effect on DNA synthesis of fetal lung cells. [4] SF 6847 (1 μM) causes increased exon inclusion of MAPT exon 10. SF 6847 does not affect the translation or stability of the two mRNAs. SF 6847 (1.6 μM) increases the inclusion of MAPT exon 10 by 2-fold in SHSY-5Y cells. [5] | |||||
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| Features | ||||||
Protocol(Only for Reference)
Kinase Assay: [1]
| Binding assay | The cells are solubilized in 1 mL per 2×107 cells of cold solubilization buffer (50 mM Hepes buffer, pH 7.6, 1% Triton X-100, 150 mM NaCl, 5 mM EGTA, 1 mM PMSF, 50 pg/mL aprotinin, 25 mM benzamidine, 5 pg/mL leupeptin, and 10 kg/mL soybean trypsin inhibitor) for 20 min at 4 ℃. After centrifugation at 1×107g for 30 min, the supernatant is loaded onto a WGA-agarose column and shaken for 2 hours at 4 ℃. The unabsorbed material is removed and the resin is washed twice with HTN buffer (50 mM Hepes, pH 7.6, 0.1% Triton X-100, 150 mM NaCl), twice with HTN buffer containing 1 M NaCl, and twice with HTNG buffer (50 mM Hepes, pH 7.6, 0.1% Triton X-100, 150 mM NaCl, and 10% glycerol). The EGF receptor is eluted batchwise with HTNG buffer containing 0.5 M NADG. The eluted material is stored in aliquots at -70℃ and diluted before use with TMTNG buffer (50 mM Tris-Mes buffer, pH 7.6, 0.1% Triton X-100, 150 mM NaCl, 10% glycerol). |
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References
Chemical Information
| Molecular Weight (MW) | 282.38 |
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| Formula | C18H22N2O |
| CAS No. | 10537-47-0 |
| Storage | 3 years -20℃Powder |
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| 6 months-80℃in DMSO | |
| Syonnyms | Tyrphostin AG 17, Malonaben, NSC 242557, RG-50872 |
| Solubility (25°C) * | In vitro | DMSO | 56 mg/mL (198 mM) |
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| Water | <1 mg/mL (<1 mM) | ||
| Ethanol | 56 mg/mL (198 mM) | ||
| * <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. | |||
| Chemical Name | Propanedinitrile, 2-[[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methylene]- |
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Preparing Stock Solutions
| Stock Solution (1ml DMSO) | 1mM | 10mM | 20mM | 30mM |
|---|---|---|---|---|
| Mass(mg) | 0.28238 | 2.8238 | 5.6476 | 8.4714 |
