Tylosin ELISA Kit

Alternate Name :

Tag Line :

A Competitive ELISA kit for in vitro quantitative determination of Tylosin concentrations in Tissue and Honey samples

Storage Conditions: 4°C

Shelf life: 12 months

Summary :

Detection Method: Absorbance (450 nm)

Features & Benefits:

Cross-reactivity: Tylosin - 100%, Erythromycin - 1%, Other macrolides ＜1%, Sample recovery rate: Tissue - 85%±10%, Honey- 85%±15%, Detection limit: Tissue- 1 ppb, Honey- 0.5 ppb, Sensitivity: 0.5 ppb (ng/mL)

Description:

Tylosin is an antibiotic and a bacteriostatic feed additive used in veterinary medicine. It has a broad spectrum of activity against Gram-positive organisms and a limited range of Gram-negative organisms. It is found naturally as a fermentation product of Streptomyces fradiae. It is a macrolide antibiotic. Tylosin is used in veterinary medicine to treat bacterial infections in a wide range of species and has a high margin of safety. It has also been used as a growth promotant in some species, and as a treatment for colitis in companion animals. Tylosin ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Tylosin. During the reaction, Tylosin in the samples or standard competes with Tylosin coated on the plate for binding to the anti-Tylosin antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Tylosin. The concentration of Tylosin in the samples can be calculated by comparing the OD of the samples to the standard curve.