TUCAN/CARD8 Antibody

Antibody Target: TUCAN

Alternative Name: DACAR , NDPP1 , TUCAN , CARDINAL , KIAA0955 , MGC57162 , DKFZp779L0366

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding amino acid 420 of human TUCAN

Accession#: Q9Y2G2

Gene ID: 22900

Appearance: Colorless liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (0.2 mg/ml) Protein A affinity purified rabbit anti-TUCAN polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5 BSA and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human, mouse, rat

Application: Western blot

Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 47 kDa TUCAN in samples from human, mouse, and rat origins. Jurkat cell lysate, mouse small intestine and rat kidney tissue lysates can be used as positive controls.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: TUCAN (Tumor Up-regulated Card-containing Antagonist of Caspase-Nine) is a CARD (Caspase-Associated Recruitment Domains) containing protein that binds and suppresses activation of pro-Caspase-9 and found to be overexpressed in some types of cancers. TUCAN binds specifically to pro-caspase-9 and interferes with its interaction with Apaf-1, thereby suppressing apoptosis signaling in the mitochondrial/cytochrome c pathway.

Handling: The antibody solution should be gently mixed before use.