TrkB Antibody

Antibody Target: TrkB

Alternative Name: NTRK2 , GP145-TrkB , TRKB , Trk-B , tyrosine receptor kinase

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding amino acid 810 of human TrkB

Accession#: Q63604

Gene ID: 25054

Appearance: Colorless liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (0.2 mg/ml) immunoaffinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal

Purification: Affinity purified

Species Reactivity: human, mouse, rat, chicken

Application: Western blot, Immunoprecipitation, Immunofluorescence

Application & Usage: Western blotting (0.5-4 µg/ml), immunofluorescence (5-10 µg/ml). However, the optimal concentrations should be determined individually. Trk protein exists as variably glycosylated entities with the major forms having molecular weights of 140 kDa, 110 kDa, and the unglycosylated form of 80 kDa.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: Due to the various splice variants, the trk proteins exist as variably glycosylated entities with the major forms having molecular weights of 140-145 kDa, 110 kDa and the unglycosylated form of 80 kDa. trkB contains 33.3% carbohydrate by weight representing modification on 10 of 12 N-glycosylation sites. The primary ligand for trkB is BDNF which induces the phosphorylation of the protein and subsequent binding of PLC-gamma via SH2 domains. TrkB may function to modulate neuronal responses to the neurotrophins acting through trkB such as BDNF.

Handling: The antibody solution should be gently mixed before use.