TrkA Antibody

Antibody Target: TrkA

Alternative Name: Trk-A, TRK1 , NTRK1, TRK, CIPA

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding amino acid 494 of human TrkA

Accession#: BAA34355

Gene ID: 4914

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-TrkA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human, mouse, rat

Application: Immunoprecipitation, Immunohistochemistry

Application & Usage: Western blotting (0.5-4 µg/ml) in immunoprecipitation, and Immunohistochemistry. However, the optimal conditions should be determined individually. The antibody detects ~140 kDa TrkA. It does not cross-react with TrkB or TrkC.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: TrkA, the high affinity Nerve Growth Factor (NGF) receptor, autophosphorylates on tyrosine to activate multiple effectors. Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects Trk kinase activity.

Handling: The antibody solution should be gently mixed before use.