Triglyceride Quantification Colorimetric/Fluorometric Kit
Kits
Description
Triglycerides (TG) are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of
fatty acids as well as serving as an energy source. TG are broken down into fatty acids and glycerol, after which both can serve as
substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and
stroke as well as in pancreatitis. The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a
variety of samples. In the assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a product
which reacts with the probe to generate color (spectrophotometry at λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can
detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.
fatty acids as well as serving as an energy source. TG are broken down into fatty acids and glycerol, after which both can serve as
substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and
stroke as well as in pancreatitis. The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a
variety of samples. In the assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a product
which reacts with the probe to generate color (spectrophotometry at λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can
detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.