TRAIL/Apo2L, human recombinant

Alternate Names / Synonyms: Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, Apo-2 ligand, Apo-2L, CD253 antigen, TL2, APO2L, TNFSF10.

Gene Symbol: TNF10

Accession #: P50591

Gene ID: 8743

Gene Source: Human

Recombinant Host: E. coli

Appearance: Lyophilized protein

Physical Form: Sterile filtered and lyophilized

Molecular Weight: 19.6 kDa

Purity by SDS-PAGE: ≥98%

Purity by HPLC: ≥98%

Endotoxin Level: <0.1 ng/μg

Biological Activity: The activity is determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The EDâ‚…â‚€ for the effect is 1-3 ng/ml.

Reconstitution: Reconstitute in water to a concentration of 0.5-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Storage Temp.: -20°C

Shipping: Gel pack

Background Information: Human TRAIL (TNF-Related Apoptosis Inducing Ligand), also called Apo2 Ligand (Apo2L), is a cytotoxic protein that activates rapid apoptosis in tumor cells, but not at normal cells. The recombinant human TRAIL/Apo2L is a single polypeptide chain containing 168 amino acids and has a molecular mass of 19.6 kDa.

Handling: Centrifuge the vial prior to opening.