Proteins and Peptides
TPSAB1 Antibody (LS-B2478)
- 貨號 Cat. No.
- LS-B2478
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2478 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C25456. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Tryptase Alpha/beta 1 (TPSAB1) |
| Synonyms: | TPSAB1, beta-tryptase, mast cell tryptase beta, MGC104064, TPS1, Tps2, TPSB1, Tryptase beta, Tryptase beta I, tryptase beta II, tryptase beta III, Tryptase-1, Tryptase-I, Tryptase-III |
| Family: | Protease |
| Subfamily: | Serine S1 |
| Host: | TPSAB1 antibody was produced in Mouse |
| Antigen Species: | TPSAB1 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antigen Type: | Purified protein |
| Immunogen: | TPSAB1 antibody was raised against human mast cell tryptase purified from human lung tissue. |
| Antibody Specificity: | Recognizes human mast cell tryptase, both alpha and beta isoforms. The antibody is an excellent marker for mast cells and does not bind to any other cell type in Immunohistochemistry. Species cross-reactivity: feline, canine and monkey. |
| Predicted Species Reactivity: | Cat, Dog, Monkey |
| Purification: | Protein G Column |
| Presentation: | PBS, pH 7.4, 0.09% sodium azide |
| Recommended Storage: | Long term: Add glycerol (40-50%) -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2478 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2478 was determined to be 5 ug/ml. Positive control: Tonsil. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr), Western blot (WB), ELISA (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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