TPO, murine recombinant

Alternate Names / Synonyms: Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.

Gene Symbol: TPO

Accession #: P40226

Gene ID: 21832

Gene Source: Mouse

Recombinant Host: E. coli

Appearance: Lyophilized protein

Physical Form: Sterile filtered and lyophilized from 10 mM Tris, pH 8.0

Molecular Weight: 18.7 kDa

Purity by SDS-PAGE: ≥98%

Purity by HPLC: ≥98%

Endotoxin Level: <0.1 ng/μg

Biological Activity: The EDâ‚…â‚€ as determined by the dose-dependent stimulation of the proliferation of human MO7e cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.

Reconstitution: Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Further dilutions of this solution should be made into buffer containing carrier protein or medium containing serum. This solution can be stored at 4°C for 1 week or –20°C for future use.

Storage Temp.: -20°C

Shipping: Gel pack

Background Information: TPO (Thrombopoietin, also known as Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulation levels of platelets in vivo. The murine TPO is a soluble 18.7 kDa protein containing 174 amino acid residues, which comprises the receptor binding domain of the Mpl-ligand protein.

Handling: Centrifuge the vial prior to opening.