TP53 Antibody (LS-B2232)

貨號 Cat. No.: LS-B2232
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2232
Antibody Validation:	This antibody replaces catalog number LS-C25891. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Tumor Protein P53 (Li-Fraumeni Syndrome) (TP53)
Synonyms:	TP53, cellular tumor antigen p53, p53, p53 tumor antigen , tp53, tumor protein p53
Family:	P53
Subfamily:	Not assigned-Other
Host:	TP53 antibody was produced in Rabbit
Antigen Species:	TP53 antibody was raised against Human
Clonality:	Polyclonal
Antigen Type:	Recombinant protein
Immunogen:	TP53 antibody was raised against recombinant human full-length wild type p53 protein. Cellular Localization: Nuclear
Antibody Specificity:	Recognizes human p53. Species cross-reactivity: mouse and rat.
Predicted Species Reactivity:	Mouse, Rat
Purification:	Immunoaffinity Chromatography
Presentation:	10 mM PBS, pH 7.6, 0.2% BSA, 15 mM sodium azide, 40% glycerol.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2232 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2232 was determined to be 5 ug/ml. Western Blot: 2-4 ug/ml at RT for 2 hours. Positive control: SKBR-3 or MDA-231 cells. Colon carcinomas.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µl