Total Bile Acids Assay (Enzyme Cycling Method)

The Diazyme Total Bile Acids (TBA) Assay is a liquid stable system, ready to use for both manual methods and is adaptable for many automated chemistry analyzers. The assay has excellent sensitivity with a linear range from 0 - 180 μM. Diazyme's unique enzyme cycling method amplifies signal for accurate and fast measurement using a small sample up to 50% less than conventional NBT methods making it an excellent choice. The assay has excellent precision with Intra-Assay Precision CV% of <4% and Inter-Assay Precision CV% of <3%. The following substances normally present in serum produced less than 10% deviation at the listed concentrations: Triglycerides at 750 mg/dL, Ascorbic acid at 50 mg/dL, Bilirubin at 50 mg/dL and Hemoglobin at 500 mg/dL.