TOP2A Antibody (LS-B2408)

貨號 Cat. No.: LS-B2408
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2408
Antibody Validation:	This antibody replaces catalog number LS-C26362. It has been validated for use in the following assays: IHC-P.
Antibody Target:	TOP2A
Synonyms:	TOP2A Antibody, dna topoisomerase ii, alpha isozyme Antibody, TOP2 Antibody, TP2A Antibody
Family:	Topoisomerase
Subfamily:	Not assigned
Host:	TOP2A antibody was produced in Rabbit
Antigen Species:	TOP2A antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	TOP2A antibody was raised against a synthetic peptide corresponding to C-terminal residues of human Topo II .
Antibody Specificity:	Recognizes human Topo II.
Antigen Modification:	C-Terminus
Predicted Species Reactivity:	Mouse, Rat
Purification:	Immunoaffinity Chromatography
Presentation:	50 mM Tris-glycine, pH 7.4, 0.18 M sodium chloride, 0.25% sodium azide, 0.25 mM EDTA, 25 mM citric acid, 0.5% BSA, 40% glycerol.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2408 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2408 was determined to be 1:100.
Antibody Uses:	Immunohistochemistry (IHC) (1:100 - 1:250), Immunohistochemistry - Paraffin (IHC-P) (1:100), Immunocytochemistry (ICC), Western blot (WB) (1:20000), Immunoprecipitation (IP) (1:50), Flow Cytometry (Flo) (Optimal dilution to be determined by the researcher)
Size:	50 µl