TNF-R1 Antibody

 Antibody Target: TNF-RI

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding amino acid 32 of human TNF-RI

Accession#: P25118

Gene ID: 21937

Appearance: Colorless liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (0.2 mg/ml) affinity purified rabbit anti-TNF-R1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt/ht/bv/rb/mk

Application: WB/IHC

Application & Usage: Western blotting (0.5-4 µg/ml) and in immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects cdc42 of human, mouse, rat, and bovine origins.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: Tumor necrosis factor receptor 1 and 2 (TNF-R1 and TNF-R2) are 55 and 75 kDa. While TNF-R1 and TNF-R2 share 28% sequence homology in the extracellular domains, their intracellular domains lack sequence homology, suggesting that they differ in their internal signal transduction pathways. TNF-R1 contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF-R1 associated death domain protein), and subsequent interactions with FADD. TNF-R1 can also activate the transcription factor NFkB via TRAF2 (TNF receptor associated factor 2). The cytoplasmic domain of TNF-R1 can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade.