Proteins and Peptides
TNF Antibody (LS-B2574)
- 貨號 Cat. No.
- LS-B2574
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2574 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C43649. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Tumor Necrosis Factor (TNF) |
| Synonyms: | TNF, Cachectin, DIF, tnf alpha, TNF-a, TNFA, TNFSF2, tumor necrosis factor alpha |
| Family: | TNF |
| Subfamily: | not assigned-TNF |
| Host: | TNF antibody was produced in Rat |
| Antigen Species: | TNF antibody was raised against Mouse |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antibody Clone Name: | MP6-XT22 |
| Modifications: | Low Endotoxin (Low Endotoxin) |
| Antigen Type: | Recombinant protein |
| Immunogen: | TNF antibody was raised against recombinant murine TNF alpha expressed in E. coli |
| Antibody Specificity: | Specific for mouse tumor necrosis factor alpha (TNF alpha), a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immuno-regulatory role. Clone MP6-XT22 is reported to neutralize the effects of mouse TNF alpha (4,5) |
| Purification: | Protein G Column |
| Presentation: | PBS, pH 7.4. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2574 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2574 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilization is required for this application. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Immunohistochemistry - Frozen (IHC-Fr), Flow Cytometry (Flo) (1:50 - 1:100), ELISA (1 - 10 µg/ml), Functional Assay (Func) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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