TMS1 Polyclonal Ab

HOST: Rabbit

FORMULATION: 100 ug (0.5 mg/ml) rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.

SPECIES REACTIVITY: human

STORAGE CONDITIONS: Store at -20oC. For long term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles.

BACKGROUND: TMS1 (target of methylation-induced silencing) is a recently identified gene that is aberrantly methylated and silenced in human breast cancer cells. Forty percent (11 of 27) of primary breast tumors exhibited aberrant methylation of TMS1. Ectopic expression of TMS1 induced apoptosis in 293 cells and inhibited the survival of human breast cancer cells. TMS1 encodes a 22-kDa predicted protein containing a COOH-terminal caspase recruitment domain (CARD). However, it is structurally unrelated to other known CARD adaptor and regulatory proteins. Ectopic expression of TMS1 alone was able to trigger apoptosis in 293 cells, and cell death correlated with relocalization of TMS1 from the cytoplasm to perinuclear, ball-like structures.

SPECIFICITY: The antibody recognizes human TMS1. Reactivity to other species has not been tested.

APPLICATION: The antibody can be used in Western blotting (1-4 ug/ml). However, the optimal concentrations should be determined individually.

USAGE: For Research Use Only. Not to be used in human.