Proteins and Peptides
TLR9 Antibody (LS-B2839)
- 貨號 Cat. No.
- LS-B2839
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2839 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C10779. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | TLR9 |
| Synonyms: | TLR9 Antibody, CD289 Antibody, scri2a Antibody, tlr9 Antibody, toll-like receptor 9 Antibody, toll-like receptors 9 Antibody |
| Family: | Toll-like Receptor |
| Subfamily: | not assigned-Toll-like Receptor |
| Host: | TLR9 antibody was produced in Mouse |
| Antigen Species: | TLR9 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Modifications: | Fluorescein (FITC) |
| Antigen Type: | Synthetic peptide |
| Immunogen: | TLR9 antibody was raised against synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9). |
| Antibody Specificity: | Recognizes human TLR9. Detects a band of ~120kD by Western blot, may also detect a smaller band of TLR9 isoform B. Species cross-reactivity: mouse, rat, equine, canine, and monkey. |
| Antigen Modification: | aa268-284 |
| Predicted Species Reactivity: | Canine, Equine, Monkey, Mouse, Rat |
| Purification: | Protein G Column |
| Presentation: | 10 mM Tris, 150 mM sodium chloride, 0.05% sodium azide. |
| Recommended Storage: | Short term +4C. Long term add glycerol to 40-50%. Aliquot to avoid freeze/thaw cycles and store at -20C. FITC conjugates are sensitive to light. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2839 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2839 was determined to be 5-10 ug/ml. Flow Cytometry (Intracellular): 0.1-2 ug/10^6 cells; cell surface. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 - 10 µg/ml), Immunohistochemistry - Frozen (IHC-Fr) (10 - 20 µg/ml), Western blot (WB) (1 - 3 µg/ml), Flow Cytometry (Flo) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |
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