TLR4 Antibody

Antibody Target: TLR4

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Recombinant human TLR4

Accession#: O00206

Gene ID: 7099

Appearance: Colorless liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (0.2 mg/ml) Protein A affinity purified rabbit anti-TLR4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purification: Affinity purified

Species Reactivity: hu

Application: WB/FC

Application & Usage: Western blot analysis (0.5-4 µg/ml). The antibody is also suitable for flow cytometric analysis of cell surface expression of TLR4. However, the optimal conditions should be determined individually.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: TLR4 (Toll-like receptor 4) is a mammalain homologue of the Drosophila toll protein. The human TLR4 is a type-1 transmembrane protein containing 799 amino acid residues. The extracellular domain has leucine-rich repeats and the intracellular domain is similar to that of IL-1 receptor. TLR4 is a receptor for lipopolysaccharide (LPS). In mice that are genetically tolerant to endotoxin, mutations have been identified in the intracellular domain of TLR4 that abolish LPS responsiveness. TLR4 uses a mechanism similar to that of the IL-1 receptor for signal transduction that leads to activation of NF-kB.