TLR2 Antibody

Antibody Target: TLR2

Host: Mouse

Antibody Type: Monoclonal

Isotype: Mouse IgG1

Antibody Form: Affinity Purified Antibody

Immunogen: Recombinant human TLR2

Accession#: O60603

Gene ID: 7097

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µl protein G purified mouse anti-human TLR2 monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purification:

Species Reactivity: hu

Application: WB

Application & Usage: Western blotting (1:200-1000 dilution). However, the optimal conditions should be determined individually. Ramos cell lysate can be used as a positive control and a ~86 kDa band is detected.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: The Toll-like receptor (TLR) family in mammalian comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Up-to-date, ten TLRs (TLR1-10) have been described. TLR2 is differentially expressed in human cells. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells. CD3+ T-cells and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulate TLR2, whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.