Tissue Cell Surface Antigen Immunofluorescent Staining Kit 組織細胞表é¢æŠ—原螢光染色試劑
- 貨號 Cat. No.
- FC023
- 品牌 Brand
- gene-rl
Description
組織細胞表é¢æŠ—原螢光染色試劑
Tissue Cell Surface Antigen Immunofluorescent Staining Kit
(Cat. No. FC023)
Introduction:
Flow cytometry requires the preparation of suspension of single cells. While it is comparatively easy with blood cells or cultured cells, the dispersion of intact cells from solid tissues is virtually impossible because cell junctions are very difficult to disrupt without damage to the cells membrane. We introduce this techniques that base on buffer with citric acid treatment that is applied either to fresh tissue samples or to archival material embedded in paraffin.
When using whole blood cell or spleen cell suspensions for flow cytometric analysis, it is recommended to remove red blood cells. The kit is intended for lysing erythrocytes in single cell suspensions of hematopoietic tissues such as spleen and human peripheral blood. The buffer lysis red cells with minimal effect on lymphocytes which following direct immuno- fluorescence staining monoclonal antibodies prior to flow cytometric analysis. The cells may be stored for a month before analysis.
Contents of the kit:
Items | Quantity (100 tests) | Storage conditions | Dilute notice |
| Dis-aggregation buffer (FC0011) | 1 bottle (120 ml) | 4 ℃ | |
| 10X Wash buffer(FC0022) | 1 bottle (250 ml) | 25 ℃ | ＊ |
| Fix buffer(FC0023) | 1 bottle (120 ml) | 4 ℃ | |
| 10x RBC Lysis Buffer (FC0021) | 1 bottle (50 ml) | 4 ℃ | ＊ |
| Meshes | 100 tests | 25 ℃ |