Proteins and Peptides
Thrombospondin Antibody (LS-B2570)
- 貨號 Cat. No.
- LS-B2570
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2570 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C26351. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Thrombospondin |
| Host: | Thrombospondin antibody was produced in Mouse |
| Antigen Species: | Thrombospondin antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antibody Clone Name: | A6.1 |
| Modifications: | Azide-free (Azide-free) |
| Antigen Type: | Purified protein |
| Immunogen: | Thrombospondin antibody was raised against reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP |
| Antibody Specificity: | Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat. |
| Predicted Species Reactivity: | Bovine, Dog, Horse, Mouse, Porcine, Rat, Sheep |
| Purification: | Protein G Column |
| Presentation: | 10 mM PBS, pH 7.4. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2570 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2570 was determined to be 10 ug/ml. Immunoprecipitation: 2 ug/mg protein lysate. Use Protein G. Western Blot: 1-2 ug/ml for 2 hrs at RT. Positive control: Platelets in placenta, megakaryocytes in bone marrow. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB), Immunoprecipitation (IP), Flow Cytometry (Flo), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 1 mg/ml |
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