Antibodies
Thiazovivin
- 貨號 Cat. No.
- S1459
- 規格 Size
- 5~200mg
- 品牌 Brand
- Selleckchem
Description
Thiazovivin
Thiazovivin (Tzv) is a novel ROCK inhibitor with IC50 of 0.5 μM, promotes hESC survival after single-cell dissociation.
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Distributor Tel: +886-2-2827-1197jimmy@new.abscience.com.tw
Biological Activity
| Description | Thiazovivin (Tzv) is a novel ROCK inhibitor with IC50 of 0.5 μM, promotes hESC survival after single-cell dissociation. | |||||
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| Targets | ROCK | |||||
| IC50 | ~0.5 μM [1] | |||||
| In vitro | Although displaying little impact on cell proliferation, Thiazovivin treatment significantly enhances the survival of human embryonic stem cells (hESCs) after enzymatic dissociation more than 30-fold, while homogenously maintaining pluripotency with the characteristic colony morphology, expression of typical pluripotency markers such as alkaline phosphatase (ALP), and normal karyotype. Dissociated hESCs treated with Thiazovivin display dramatically increased adhesion to matrigel- or laminin-coated plates but not to gelatin-coated plates within a few hours. Thiazovivin treatment increases cell-ECM adhesion-mediated β1 integrin activity, which synergizes with growth factors to promote cell survival. In addition to activating integrin, Thiazovivin but not Tyrintegin (Ptn) protects hESCs from death in the absence of ECM in suspension through E-cadherin-mediated cell-cell interaction. Thiazovivin treatment potently inhibits endocytosis of E-cadherin, consequently stabilizing E-cadherin on the cell surface and leading to reestablishment of cell-cell interaction, which is essential for hESC survival in ECM-free conditions. Thiazovivin but not Tyrintegin (Ptn) at 2 μM inhibits Rho-associated kinase (ROCK) activity and protects hESCs at a similar level as the widely used selective ROCK inhibitor Y-27632 at 10 μM, suggesting that Rho-ROCK signaling regulates cell-ECM and cell-cell adhesion. [1] Thiazovivin at 1 μM increases the reprogramming efficiency of CB mononuclear cells to induced pluripotent stem cells (iPSCs) by more than 10 times. [2] | |||||
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Protocol(Only for Reference)
Kinase Assay:
| In vitro ROCK assay | Thiazovivin is dissolved in DMSO. CycLex Rho-kinase assay kit is used to detect ROCK activity using recombinant ROCK in the presence of increasing concentrations of Thiazovivin (~10 μM). |
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References
Chemical Information
| Molecular Weight (MW) | 311.36 |
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| Formula | C15H13N5OS |
| CAS No. | 1226056-71-8 |
| Storage | 3 years -20℃Powder |
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| 6 months-80℃in DMSO | |
| Syonnyms | N/A |
| Solubility (25°C) * | In vitro | DMSO | 15 mg/mL (48 mM) |
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| Water | <1 mg/mL (<1 mM) | ||
| Ethanol | <1 mg/mL (<1 mM) | ||
| In vivo | 30% PEG400/0.5% Tween80/5% propylene glycol, | 30 mg/mL | |
| * <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. | |||
| Chemical Name | N-benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide |
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Preparing Stock Solutions
| Stock Solution (1ml DMSO) | 1mM | 10mM | 20mM | 30mM |
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| Mass(mg) | 0.31136 | 3.1136 | 6.2272 | 9.3408 |
Customer Reviews (1)
 Click to enlarge | Rating | |
| Source | Arch Toxicol. 2013 Dec;87(12):2215-31 . Thiazovivin purchased from Selleck | |
| Method | Cellomics Array Scan | |
| Cell Lines | LUHMES human neuronal precursor cells | |
| Concentrations | ||
| Incubation Time | 24 h | |
| Results | Thiazovivin accelerated neurite growth significantly. |
Product Citations (3)
Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes. [Yang H, et al. Cell Res 2013;23(10):1187-200]
Evaluation of a human neurite growth assay as specific screen for developmental neurotoxicants. [Krug AK , et al. Arch Toxicol 2013;87(12):2215-31]
Induced Pluripotent Stem Cells Generated from Adult Bone Marrow-Derived Cells of the Nonhuman Primate (Callithrix jacchus) Using a Novel Quad-Cistronic and Excisable Lentiviral Vector. [Wiedemann A, et al. Cell Reprogram 2012;14(6):485-96]
