Proteins and Peptides
THBS1 Antibody (LS-B2156)
- 貨號 Cat. No.
- LS-B2156
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2156 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C26345. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Thrombospondin 1 (THBS1) |
| Synonyms: | THBS1, thrombospondin 1 |
| Host: | THBS1 antibody was produced in Mouse |
| Antigen Species: | THBS1 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antigen Type: | Purified protein |
| Immunogen: | THBS1 antibody was raised against purified human TSP. |
| Antibody Specificity: | Designed for sensitive detection of TSP by Western Blot. It shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Species cross-reactivity: human. |
| Purification: | Protein G Column |
| Presentation: | 10 mM PBS, pH 7.4. |
| Recommended Storage: | Long term: Add glycerol (40-50%) aliquot and store at -20C; Short term:+4C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2156 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2156 was determined to be 5 ug/ml. Western Blot: Reduced and non-reduced. 0.5-1.0 ug/ml for 2 hours at RT. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 1 mg/ml |
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