TH Antibody (LS-B2462)

貨號 Cat. No.
LS-B2462
規格 Size
50 µl
品牌 Brand
LSBio

Description
















































































Antibody Catalog ID: LS-B2462
Antibody Validation: This antibody replaces catalog number LS-C232. It has been validated for use in the following assays: IHC-P.
Antibody Target: TH
Synonyms: TH Antibody, DYT14 Antibody, DYT5b Antibody, TYH Antibody
Family: Monooxygenase
Subfamily: Not assigned
Host: TH antibody was produced in Rabbit
Antigen Species: TH antibody was raised against Rat
Clonality: Polyclonal
Immunogen: TH antibody was raised against phosphopeptide corresponding to amino acid residues surrounding the phosphoSer40 of rat tyrosine hydroxylase
Antibody Specificity: Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.
Antigen Modification: pSer40
Predicted Species Reactivity: Mammal
Purification: Immunoaffinity Chromatography
Presentation: 50 uL of 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50% glycerol.
Recommended Storage: +4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary: Immunohistochemistry: LS-B2462 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2462 was determined to be 5 ug/ml.
Antibody Uses: Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr) (1:1000), Western blot (WB) (1:1000), Immunofluorescence (IF) (1:1000) (Optimal dilution to be determined by the researcher)
Size: 50 µl

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