Proteins and Peptides
TGM2 Antibody (LS-B2681)
- 貨號 Cat. No.
- LS-B2681
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2681 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C39836. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Transglutaminase 2 (TGM2) |
| Synonyms: | TGM2, G-ALPHA-h, GNAH, protein-glutamine gamma-glutamyltransferase, TG(C), TG2, TGase 2, Tgase C, Tgase II, TGase-H, tgc, Tissue transglutaminase, TRANSGLUTAMINASE 2, TRANSGLUTAMINASE C, tTG |
| Host: | TGM2 antibody was produced in Rabbit |
| Antigen Species: | TGM2 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Recombinant protein |
| Immunogen: | TGM2 antibody was raised against recombinant full length human TGase II (77-85kD). Cellular Localization: Cytoplasm, cell membrane, and extracellular matrix. |
| Antibody Specificity: | Recognizes Transglutaminase II. Species cross-reactivity: Human, mouse, rat. |
| Predicted Species Reactivity: | Mouse, Rat |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.4. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2681 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2681 was determined to be 5 ug/ml. Immunoprecipitation (Native verified): 10 ug/mg protein lysate. Use Protein A. Western Blot: 5-10 ug/ml for 2hrs at RT. Positive control: HUVEC cells. Breast carcinoma. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (5 - 10 µg/ml), Immunoprecipitation (IP) (10 µg/mg lysate) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 1 mg/ml |
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