TGF-beta3 Polyclonal Antibody

HOST: Goat

FORMULATION: 100 ug (0.5 mg/ml) antigen affinity purified goat polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.

SPECIES REACTIVITY: human, mouse, rat

STORAGE CONDITIONS: Store at -20oC. For long term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles.

BACKGROUND: The three mammalian isoforms of TGF-beta (TGF-beta1, TGF-beta2, TGF-beta3) signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and would healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-beta2 has been shown to exert suppressive effects on IL-2 dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development.

SPECIFICITY: The antibody recognizes TGF-?3 of human, mouse, and rat origins. Reactivity to other species has not been tested.

APPLICATION: The antibody can be used in Western blotting (0.5-2 ug/ml), neutralization (0.5-1 ug/ml). However, the optimal concentrations should be determined individually.

USAGE:

FOR RESEARCH USE ONLY! NOT TO BE USED IN HUMANS.