TGF-beta1, human recombinant

Alternate Names / Synonyms: Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor

Gene Symbol: TGFB1

Accession #: P01137

Gene ID: 7040

Gene Source: Human

Recombinant Host: CHO cells

Appearance: Lyophilized protein

Physical Form: Lyophilized from Sodium Citrate, pH3.5 buffer

Molecular Weight: 25.0 kDa

Purity by SDS-PAGE: ≥98%

Purity by HPLC: ≥98%

Endotoxin Level: <0.1 ng/μg

Biological Activity: The EDâ‚…â‚€ as determined by the ability of TGF-β1 to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is <0.05 ng/ml, corresponding to a specific activity of >2 x 107 units/mg.

Reconstitution: Reconstitution in water to a concentration of 50 ug/ml (e.g., 5 ug/100 ul) will yield a solution of 1 mM citrate buffer (pH 3.5). The solution can then be stored at 4°C for 1 month. It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at –20°C.

Storage Temp.: -20°C

Shipping: Gel pack

Background Information: TGF-β1 (Transforming Growth Factor-β1) is a member of the superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF-β1 is a 25.0 kDa protein containing two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

Handling: Centrifuge the vial prior to opening.