TGF-alpha, human recombinant

Alternate Names / Synonyms: Transforming Growth Factor-α, Sacroma growth factor, TGF-type I, ETGF

Gene Symbol: TGFA

Accession #: P01135

Gene ID: 7039

Gene Source: Human

Recombinant Host: E. coli

Appearance: Lyophilized protein

Physical Form: Sterile filtered and lyophilized with no additives

Molecular Weight: 5.5 kDa

Purity by SDS-PAGE: ≥98%

Purity by HPLC: ≥98%

Endotoxin Level: <0.1 ng/μg

Biological Activity: The EDâ‚…â‚€ as determined by the dose-dependent stimulation of proliferation of Balb-c 3T3 cells is <0.2 ng/ml, corresponding to a specific activity of >5 x 106 units/mg.

Reconstitution: Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use. For most in vitro applications, TGF-α exerts its biological activity in the concentration range of 0.5 to 25 ng/ml.

Storage Temp.: -20°C

Shipping: Gel pack

Background Information: Transforming Growth Factor-α (TGF-α) stimulates the proliferation of a wide range of epidermal and epithelial cells. TGF-α has been shown to play a role in the autocrine growth of certain transformed cells. Human TGF-α is a 5.5 kDa protein containing 50 amino acid residues.

Handling: Centrifuge the vial prior to opening.