TF / Transferrin Antibody (LS-B2447)

貨號 Cat. No.: LS-B2447
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2447
Antibody Validation:	This antibody replaces catalog number LS-C39807. It has been validated for use in the following assays: IHC-P.
Antibody Target:	TF / Transferrin
Synonyms:	TF Antibody, DKFZp781D0156 Antibody, PRO1557 Antibody, PRO2086 Antibody, serotransferrin Antibody
Host:	TF antibody was produced in Rabbit
Antigen Species:	TF antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Purified protein
Immunogen:	TF antibody was raised against transferrin (Human Serum)
Antibody Specificity:	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum, purified and partially purified transferrin (human serum). Cross-reactivity against transferrin from other tissues and species may occur by have not been tested.
Purification:	Ion Exchange Chromatography
Presentation:	PBS, pH 7.2, 0.01% sodium azide, 40% glycerol.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2447 was determined to be 5 ug/ml. ELISA: 1:40000-1:160000 (Coat with 1 ug transferrin in a standard sandwich ELISA using peroxidase conjugated rabbit IgG and ABTS as a substrate for 30 min at RT).
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB), Immunoprecipitation (IP), ELISA (1:40000 - 1:160000) (Optimal dilution to be determined by the researcher)
Size:	50 µg